Coding

Part:BBa_M33350:Design

Designed by: Sunny Kim   Group: Stanford BIOE44 - S010   (2010-06-06)

RBS+CDS for TodS protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


Design Notes

We added restriction digestion sites and made 3 silent mutations, 1 for EcoRI and 2 for PstI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA and added a strong ribosome binding site (BBa_B0034).

Source

The sequence was found at http://www.ncbi.nlm.nih.gov/nuccore/Y18245.1 (TodS coordinates: 11292..14228).

References

J. Lacal, M.E. Guazzaroni, P. Gutierrez-del-Arroyo, A. Busch, M. Vélez, T. Krell and J.L. Ramos, Two levels of cooperativeness in the binding of Todt to the tod operon, J Mol Biol 384 (2008), pp. 1037–1047.